Insertion junction: LMJ.RY0402.077554_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.077554
Chromosome:chromosome 8
Location:1995593
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g367950 FAP323 (1 of 12) IPR000595//IPR018490 - Cyclic nucleotide-binding domain // Cyclic nucleotide-binding-like; Cyclic nucleotide-monophosphate binding domain Flagellar Associated Protein 323 CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGAACGTACACACACGCATACACACTCAG
Internal bar code:AACATTTCGTATCGTGCCAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:253
LEAP-Seq percent confirming:74.7292
LEAP-Seq n confirming:1242
LEAP-Seq n nonconfirming:420
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CACACAGACACACACTCCCC
Suggested primer 2:CCTCTTCATCTTCCCCTTCC