Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.077554 |
Chromosome: | chromosome_8 |
Location: | 1995593 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g367950 | antisense | CDS/intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGAACGTACACACACGCATACACACTCAG |
Internal bar code: | AACATTTCGTATCGTGCCAGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 253 |
LEAP-Seq percent confirming: | 74.7292 |
LEAP-Seq n confirming: | 1242 |
LEAP-Seq n nonconfirming: | 420 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACACAGACACACACTCCCC |
Suggested primer 2: | CCTCTTCATCTTCCCCTTCC |