| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077571 |
| Chromosome: | chromosome_16 |
| Location: | 6895432 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g676533 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACATCTCCGCATCTGCCACCAGCTGCCGAC |
| Internal bar code: | AGGAGGGCGAGGGCGCGGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 123 |
| LEAP-Seq percent confirming: | 47.5356 |
| LEAP-Seq n confirming: | 434 |
| LEAP-Seq n nonconfirming: | 479 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGGACAGACGACGATGA |
| Suggested primer 2: | ACGCACACACACACACACAC |