Insertion junction: LMJ.RY0402.077586_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.077586
Chromosome:chromosome 2
Location:5789376
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g110750 LHCB7,LHCQ Light-harvesting chlorophyll a-b binding protein, chloroplast precursor; (1 of 1) K14172 - light-harvesting complex II chlorophyll a/b binding protein 7 (LHCB7) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTAGTAATACTAGGGTTCACGGGTGGCCT
Internal bar code:CAGCACAGGTGGGGTTATTCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:408
LEAP-Seq percent confirming:97.5407
LEAP-Seq n confirming:2459
LEAP-Seq n nonconfirming:62
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTCACGCTCATTGACATGCT
Suggested primer 2:GCCCCTACCTTTCCTGATTC