Insertion junction: LMJ.RY0402.077591_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.077591
Chromosome:chromosome 13
Location:468698
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g564900 MRP3 (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase; ABC transporter, multidrug resistance associated protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCGCACGGTGCCGTTGAACATGAAGGGA
Internal bar code:ACTGGGTTTGCGCCAGCGGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:225
LEAP-Seq percent confirming:1.35542
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:655
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTTGGTGCTGATTACTGA
Suggested primer 2:CACGCGAAAATGAGCTACAA