| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077610 |
| Chromosome: | chromosome 16 |
| Location: | 3870389 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690000 | HPAT2,CGL147 | Hydroxyproline O-arabinosyl transferase 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCATGCCGTGCCAACATTGATCGTCGGTT |
| Internal bar code: | CGACCATCATAAGCATGACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 658 |
| LEAP-Seq percent confirming: | 98.2562 |
| LEAP-Seq n confirming: | 5522 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACACCTCACCTACCCCTGC |
| Suggested primer 2: | CAGAAATAGTTGGCGGGAAA |