| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077617 |
| Chromosome: | chromosome 1 |
| Location: | 2010507 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010832 | (1 of 1) PTHR12411:SF389 - CATHEPSIN L1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGCGGCATGGTTAGCTAGGCACGCAGCA |
| Internal bar code: | GTCCGAGCCTGCTGCATCTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 510 |
| LEAP-Seq percent confirming: | 98.5282 |
| LEAP-Seq n confirming: | 5757 |
| LEAP-Seq n nonconfirming: | 86 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTTCTACCTGAGCACCG |
| Suggested primer 2: | AGCAGTAGCTGTGGCATCCT |