Insertion junction: LMJ.RY0402.077618_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.077618
Chromosome:chromosome 14
Location:2567577
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g625625 FTSH11 FtsH-like membrane ATPase/metalloprotease; (1 of 2) IPR000642//IPR003593//IPR005936//IPR011704//IPR027417 - Peptidase M41 // AAA+ ATPase domain // Peptidase, FtsH // ATPase, dynein-related, AAA domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAACCACCCTAACCAACCCAACCAACCCA
Internal bar code:TGGGGAGGCGCGCGCGTAATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:79
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:176
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACACACGG
Suggested primer 2:CTTGGGCTCACATAACGGAT