| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.077628 |
| Chromosome: | chromosome 13 |
| Location: | 3780345 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589700 | VPS20 | Subunit of ESCRT-III complex; (1 of 1) K12195 - charged multivesicular body protein 6 (CHMP6, VPS20) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGATCGCACATGTGGTGCCGTTGCCGA |
| Internal bar code: | AAGCGAAAGCCAGAGTGGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 465 |
| LEAP-Seq percent confirming: | 78.6611 |
| LEAP-Seq n confirming: | 1128 |
| LEAP-Seq n nonconfirming: | 306 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAATACATTGAGACGGCGG |
| Suggested primer 2: | CTGTTGCGATTGTTGCTGTT |