| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.077635 |
| Chromosome: | chromosome 11 |
| Location: | 1172162 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467688 | (1 of 1) 1.2.3.7 - Indole-3-acetaldehyde oxidase / Indoleacetaldehyde oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCACCTGGATGACGTCTGTGCCCTGGCA |
| Internal bar code: | AGTGCGGGGCGGGAGATGGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 159 |
| LEAP-Seq percent confirming: | 98.4432 |
| LEAP-Seq n confirming: | 1075 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGTGAGGTTGCGTGTAT |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |