Insertion junction: LMJ.RY0402.077635_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.077635
Chromosome:chromosome 11
Location:1172172
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467688 (1 of 1) 1.2.3.7 - Indole-3-acetaldehyde oxidase / Indoleacetaldehyde oxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTCAGACAACACGGAAAGGGAAAAAGGG
Internal bar code:ACCGAACCGCGCTGTCCACTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:191
LEAP-Seq percent confirming:99.2908
LEAP-Seq n confirming:280
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGGTGAGGTTGCGTGTAT
Suggested primer 2:CGGTGTGTGTGTGTGTGTGT