| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.077640 |
| Chromosome: | chromosome 9 |
| Location: | 1932229 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395100 | MKP4 | (1 of 3) K14165 - atypical dual specificity phosphatase [EC:3.1.3.16 3.1.3.48] (K14165); Dual-specificity protein phosphatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCTGGAGTGCTTCATTTGATCCACGCA |
| Internal bar code: | CTCCTCCCTCACCGCGAGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 550 |
| LEAP-Seq percent confirming: | 99.6977 |
| LEAP-Seq n confirming: | 3298 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTATGCGACCGTATGAGG |
| Suggested primer 2: | CACATGGACAACTGGACTGG |