| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.077644 |
| Chromosome: | chromosome 6 |
| Location: | 2708989 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g270500 | (1 of 1) PF00759//PF03067 - Glycosyl hydrolase family 9 (Glyco_hydro_9) // Chitin binding domain (Chitin_bind_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATTGCCAAGGTCAAAGGGCTTCAGCTAC |
| Internal bar code: | CTGACGACGGGGAGTCGTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 725 |
| LEAP-Seq percent confirming: | 98.2206 |
| LEAP-Seq n confirming: | 3864 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACTAGAACGGACATGGG |
| Suggested primer 2: | ACTTCTTCTGTGGGTGTGGG |