| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.077648 |
| Chromosome: | chromosome 9 |
| Location: | 7674134 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g415450 | KIN17A,KIN17-1 | (1 of 32) 3.6.4.4 - Plus-end-directed kinesin ATPase / Kinesin; Kinesin motor protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGCATGGGCCTTGCTTAGTTTGGGATA |
| Internal bar code: | CTAATAATAGGGCGTTCAGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGTGTGTATGCGCGTGTC |
| Suggested primer 2: | GTGAAGATGTCCTCCACCGT |