| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077668 |
| Chromosome: | chromosome 1 |
| Location: | 2670602 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015600 | (1 of 1) PTHR23346//PTHR23346:SF7 - TRANSLATIONAL ACTIVATOR GCN1-RELATED // TRANSLATIONAL ACTIVATOR GCN1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCGGTACATGTGACGGACGGTCACAAGA |
| Internal bar code: | TAGGCTTTCAGGCCGGACCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 527 |
| LEAP-Seq percent confirming: | 76.1504 |
| LEAP-Seq n confirming: | 1539 |
| LEAP-Seq n nonconfirming: | 482 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTCAAGCCCTTCAC |
| Suggested primer 2: | TGGCTCTGCATTGTTGTCTC |