Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.077693 |
Chromosome: | chromosome_8 |
Location: | 1034528 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g362050 | FAP49 | Flagellar Associated Protein, membrane-associated | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TATCAAGCGCAAGCACAGTCAGCACAGGAC |
Internal bar code: | ACATACGATGAGTGGTGCGTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 127 |
LEAP-Seq percent confirming: | 86.3248 |
LEAP-Seq n confirming: | 101 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGGAAGGCAGCAAGTAAGG |
Suggested primer 2: | CGGATCCAGCCCTATCACTA |