Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.077693 |
Chromosome: | chromosome_8 |
Location: | 1034684 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g362050 | FAP49 | Flagellar Associated Protein, membrane-associated | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACTGACTGTGCACGCTGGATGCGTCACACA |
Internal bar code: | TCTGACGATCTCAAAGGGGAAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 405 |
LEAP-Seq percent confirming: | 99.0099 |
LEAP-Seq n confirming: | 2600 |
LEAP-Seq n nonconfirming: | 26 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGACCCTCACATTTTGCACC |
Suggested primer 2: | CGGATCCAGCCCTATCACTA |