| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.077696 |
| Chromosome: | chromosome 12 |
| Location: | 7425150 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556650 | APC4,DIV77 | (1 of 1) K03351 - anaphase-promoting complex subunit 4 (APC4); Anaphase promoting complex subunit 4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGCGAGGGTGCTTGTAGGTTGCAAATG |
| Internal bar code: | GGTGGGCCCTGCCCAAAGAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 580 |
| LEAP-Seq percent confirming: | 65.6442 |
| LEAP-Seq n confirming: | 963 |
| LEAP-Seq n nonconfirming: | 504 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTCTGGAGCTGTCGCTTC |
| Suggested primer 2: | CCTCACCTGCAGTGTCTTGA |