Insertion junction: LMJ.RY0402.077704_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.077704
Chromosome:chromosome 10
Location:237575
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g419400 (1 of 139) IPR011011 - Zinc finger, FYVE/PHD-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGATGCATGTGCAAGCAAGATTATGCGG
Internal bar code:ACGAGGTAGTTTAAGGAACCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:390
LEAP-Seq percent confirming:94.1409
LEAP-Seq n confirming:3808
LEAP-Seq n nonconfirming:237
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TCGACTCGAGAGGAGTGGAT
Suggested primer 2:TGACCACCATTCCAGTCAAA