| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.077761 |
| Chromosome: | chromosome 2 |
| Location: | 3791287 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095550 | (1 of 1) PTHR14187//PTHR14187:SF51//PTHR22908 - ALPHA KINASE/ELONGATION FACTOR 2 KINASE // SUBFAMILY NOT NAMED // MIDASIN-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCTAGTCTACGGCTGCGATGGCTGCAA |
| Internal bar code: | AGGCACAGCAAAATGTACTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 495 |
| LEAP-Seq percent confirming: | 96.988 |
| LEAP-Seq n confirming: | 1288 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGCCTTTATCAGTGCTAC |
| Suggested primer 2: | ACCCACAACTGCATCTCCTC |