Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.077776 |
Chromosome: | chromosome_10 |
Location: | 3000519 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g440950 | FAP192 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGTGTGTGTGTGCACCTTGGCGCCCTGACC |
Internal bar code: | GGTGTCGCCAGCCCAACTGCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 726 |
LEAP-Seq percent confirming: | 96.8844 |
LEAP-Seq n confirming: | 3856 |
LEAP-Seq n nonconfirming: | 124 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCTTTGTGTCTCGTCACGC |
Suggested primer 2: | TGGTACAGAGAAGTGCAGCG |