Insertion junction: LMJ.RY0402.077797_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.077797
Chromosome:chromosome 9
Location:2488081
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g391100 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAAGGTGTAAAGGTGTAGGTTGAGGATAC
Internal bar code:CTACGGGTCCCGTTCTTTAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:446
LEAP-Seq percent confirming:99.2647
LEAP-Seq n confirming:540
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTTGGCTAGGACTCACTG
Suggested primer 2:GAGCTCGCAGAGAAGCTGAT