Insertion junction: LMJ.RY0402.077798_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.077798
Chromosome:chromosome 10
Location:465627
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre10.g420950 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTCACGCGCCCCACCGCGCCTTCCACCG
Internal bar code:TCGGCCGGAGATGTTGGCAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:245
LEAP-Seq percent confirming:99.8418
LEAP-Seq n confirming:631
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGACTTCTCTTGAAGGTG
Suggested primer 2:TCTGCAAGAAGTGGACGTTG