Insertion junction: LMJ.RY0402.077802_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.077802
Chromosome:chromosome 12
Location:2812575
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g502600 SLT1 Sodium/sulfate co-transporter; (1 of 2) PF00939//PF02080 - Sodium:sulfate symporter transmembrane region (Na_sulph_symp) // TrkA-C domain (TrkA_C) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTACCCACCCGGATCCAGATTAAGATCG
Internal bar code:TGTGGCGTTCGGGTACTTGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:113
LEAP-Seq percent confirming:94.1176
LEAP-Seq n confirming:144
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TACCTTCCACGCTACCCAAC
Suggested primer 2:GTTTGATGACCACAACGCAC