| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.077802 |
| Chromosome: | chromosome 16 |
| Location: | 1081993 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649600 | PUF4 | Puf protein; (1 of 1) K14790 - nucleolar protein 9 (NOP9) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGATCGTAGGTGGACGGTGACGGTGTA |
| Internal bar code: | TCATTGGTTAATTCGCAAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 99.6881 |
| LEAP-Seq n confirming: | 9907 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAAGAAAAAGCGGAAGGC |
| Suggested primer 2: | TGACTCAAAACACCGAGCAG |