| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.077814 |
| Chromosome: | chromosome 3 |
| Location: | 2986557 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g163600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGTTGCTTCCTGCACTATCTGCTTTGCT |
| Internal bar code: | ATTCCGCTACCTGCTCGCGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 696 |
| LEAP-Seq percent confirming: | 62.8528 |
| LEAP-Seq n confirming: | 16458 |
| LEAP-Seq n nonconfirming: | 9727 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGATGTACAATCACAACGC |
| Suggested primer 2: | CGGTGAGTTGTGAGTGTGCT |