| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.077820 |
| Chromosome: | chromosome 3 |
| Location: | 144261 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144104 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAAAGTTATTGCCCACAGCAAGCATATA |
| Internal bar code: | AGTCTGTGTGGAAGACATTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 144 |
| LEAP-Seq percent confirming: | 4.43399 |
| LEAP-Seq n confirming: | 264 |
| LEAP-Seq n nonconfirming: | 5690 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTGAGAATTGGAAATGA |
| Suggested primer 2: | ACTGCGATATACTGTCCGGG |