| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.077823 |
| Chromosome: | chromosome 2 |
| Location: | 5148781 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105287 | (1 of 1) PF13821 - Domain of unknown function (DUF4187) (DUF4187) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTACGGAGCACCGCATCGAGGATTGGTTT |
| Internal bar code: | TAGGCATGATCCCTGCACGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 311 |
| LEAP-Seq percent confirming: | 99.7677 |
| LEAP-Seq n confirming: | 6873 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGTTACTCGGGTACTTCA |
| Suggested primer 2: | TAACTAATGACCCAACCCGC |