Insertion junction: LMJ.RY0402.077833_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.077833
Chromosome:chromosome 15
Location:325697
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre15.g635750 (1 of 1) IPR003613//IPR016024 - U box domain // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACATACATACGGTGCCTGCTACCCAACC
Internal bar code:CCTTGTTCCACATGGTCAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:70
LEAP-Seq percent confirming:50.5
LEAP-Seq n confirming:303
LEAP-Seq n nonconfirming:297
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GATTGATTAGGAACGGGGGT
Suggested primer 2:GCCACAACTTCGCTTCTTTC