Insertion junction: LMJ.RY0402.077834_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.077834
Chromosome:chromosome 7
Location:5699594
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g352600 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCCGCTTTAGAAGCACACCATAGCACAC
Internal bar code:GGTGTCGTTTACCAAGGTGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:554
LEAP-Seq percent confirming:51.9551
LEAP-Seq n confirming:3654
LEAP-Seq n nonconfirming:3379
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGATGAGAAGCCAGATGC
Suggested primer 2:TTAATTGTCGGATTGCTCCC