| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077837 |
| Chromosome: | chromosome 14 |
| Location: | 2342158 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623800 | NAT6 | N-acetyltransferase; (1 of 1) PTHR23091//PTHR23091:SF66 - N-TERMINAL ACETYLTRANSFERASE // N-ALPHA-ACETYLTRANSFERASE 30 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAAACACATGCTGATCTTTCAGCCGATT |
| Internal bar code: | TAGTTGTGGCGAGTGGATTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 180 |
| LEAP-Seq percent confirming: | 99.7506 |
| LEAP-Seq n confirming: | 400 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAATTCCGGAGTTTTCAGG |
| Suggested primer 2: | AGTGCCACCTCCTGAACAAC |