Insertion junction: LMJ.RY0402.077840_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.077840
Chromosome:chromosome 13
Location:4860559
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g605600 OAS1 (1 of 1) IPR018952//IPR026774 - 2'-5'-oligoadenylate synthetase 1, domain 2/C-terminal // 2'-5'-oligoadenylate synthase; 2'-5'-oligoadenylate synthetase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTCGACAATTAGCATCCCACATCACGCA
Internal bar code:GCAGCTGGGAGGGTACGCGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:387
LEAP-Seq percent confirming:99.0916
LEAP-Seq n confirming:1309
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCGGAATTGTGTGTTTCA
Suggested primer 2:GTGAGACAGGGATGGGAAGA