| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.077858 |
| Chromosome: | chromosome 10 |
| Location: | 2725072 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438300 | LTH4 | (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease; LAT2 homolog | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCTGAGCAGCAGGGTGCATGTGTTGCA |
| Internal bar code: | TCCTTGCCCGCACCGGGTTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 45 |
| LEAP-Seq percent confirming: | 99.0291 |
| LEAP-Seq n confirming: | 204 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTTTATGGGTGCGAACT |
| Suggested primer 2: | ATTGTCATGCAATGAAGGCA |