| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.077869 |
| Chromosome: | scaffold 50 |
| Location: | 10459 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre50.g761497 | (1 of 1) PTHR13890:SF0 - MAGNESIUM TRANSPORTER MRS2 HOMOLOG, MITOCHONDRIAL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGTCAATTTGTGCTCGTTTGCAACCTG |
| Internal bar code: | TCCATAGCCATCAATTTCGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 26 |
| LEAP-Seq percent confirming: | 60.2804 |
| LEAP-Seq n confirming: | 129 |
| LEAP-Seq n nonconfirming: | 85 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTATTGTACACATGCGCCG |
| Suggested primer 2: | CGTTCACACACATGGCTACC |