| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.077879 |
| Chromosome: | chromosome 11 |
| Location: | 2570159 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g476250 | (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATTTATCCACGTTGTCTAGATCCGCAAC |
| Internal bar code: | GTAATCCCAAATTCCCCACTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 212 |
| LEAP-Seq percent confirming: | 97.2376 |
| LEAP-Seq n confirming: | 528 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTCCCTTCTGCCCGAG |
| Suggested primer 2: | ACGCAGCCGCTGTACTTATT |