Insertion junction: LMJ.RY0402.077881_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.077881
Chromosome:chromosome 4
Location:3621854
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g228650 (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCACAGACCCACCTGCACCGCCAGCTGT
Internal bar code:ACGATTTCAATCTTTTCACATA

Confirmation - LEAP-Seq

LEAP-Seq distance:307
LEAP-Seq percent confirming:99.3532
LEAP-Seq n confirming:5530
LEAP-Seq n nonconfirming:36
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCAGAATCTCCCACACTG
Suggested primer 2:TACGTGTGAGGGGTCAAACA