| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077886 |
| Chromosome: | chromosome 15 |
| Location: | 733833 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640250 | (1 of 1) K07562 - nonsense-mediated mRNA decay protein 3 (NMD3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCCAATACCTCCCCCACACACACCTGCA |
| Internal bar code: | GCGCATCACTGAGAGAGGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 493 |
| LEAP-Seq percent confirming: | 97.9008 |
| LEAP-Seq n confirming: | 27703 |
| LEAP-Seq n nonconfirming: | 594 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGATTCTCCTCCTTCCC |
| Suggested primer 2: | GTTTGGTATAGGGGGAGGGA |