| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077919 |
| Chromosome: | chromosome_2 |
| Location: | 460927 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g076466 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AATGCAAATTACGTGTTGGCACGAAGTACT |
| Internal bar code: | CTAGGCTAACCTCCCTTTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 422 |
| LEAP-Seq percent confirming: | 75.2056 |
| LEAP-Seq n confirming: | 640 |
| LEAP-Seq n nonconfirming: | 211 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGGTACATCCTGAAGGT |
| Suggested primer 2: | CGACGTGGAGTTGAGCAGTA |