| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.077927 |
| Chromosome: | chromosome 12 |
| Location: | 5686882 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532500 | (1 of 2) K03453 - bile acid:Na+ symporter, BASS family (TC.BASS); Sodium:bile acid symporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGGGAGGCGGTCATTGCGTCGCAGCGCT |
| Internal bar code: | ATTACGTTGAGGTCCCTGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 119 |
| LEAP-Seq percent confirming: | 99.061 |
| LEAP-Seq n confirming: | 1899 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCTCCTGACTCAACAGA |
| Suggested primer 2: | TAATTTTATGTCACGCCGCA |