| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.077935 |
| Chromosome: | chromosome 6 |
| Location: | 2343584 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g267400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCTCCGAGTAGCGGTGATCCCGTGCGG |
| Internal bar code: | TTAGGTGCCGGGCCGTGGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 652 |
| LEAP-Seq percent confirming: | 98.7413 |
| LEAP-Seq n confirming: | 706 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTGCCTAGCAAATATCA |
| Suggested primer 2: | TGATGATGGCGATTCCACTA |