| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.077937 |
| Chromosome: | chromosome 8 |
| Location: | 263235 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358557 | (1 of 2) PF10173 - Mitochondrial K+-H+ exchange-related (Mit_KHE1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGTTGGTGTCAGCTGTGGCGTGGTCG |
| Internal bar code: | GGCTACTCGGATGGAGGTCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 588 |
| LEAP-Seq percent confirming: | 99.1714 |
| LEAP-Seq n confirming: | 1915 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCAAACTCAACCAGGACG |
| Suggested primer 2: | CCCTAGCAAGCACCAGACTC |