| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.077951 |
| Chromosome: | scaffold 36 |
| Location: | 19082 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre36.g759697 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGGGCTTTCAGCAGTGGAGGAGGAAGA |
| Internal bar code: | AGCCAGTGTCTGCCTAGCAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 99.2429 |
| LEAP-Seq n confirming: | 1442 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTGTGTTCCCTTCCCTA |
| Suggested primer 2: | GACAGTGGATTACGGCAGGT |