| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077955 |
| Chromosome: | chromosome 3 |
| Location: | 693906 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145987 | (1 of 7) IPR000104//IPR020683 - Antifreeze protein, type I // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCACACCACAGCCTTCCAGCCAACACCA |
| Internal bar code: | GCACCTCGGGTCGGAAACGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 28 |
| LEAP-Seq percent confirming: | 71.1864 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCAGACCATTCTAAGGC |
| Suggested primer 2: | ACCAGCCAAATCAGAACCAG |