| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.077961 |
| Chromosome: | chromosome 1 |
| Location: | 7041456 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050800 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTATTGCAGCGGGCGGCCGGGGAGGCGGA |
| Internal bar code: | CTCCGTAGCACACGTGGAAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 478 |
| LEAP-Seq percent confirming: | 98.5295 |
| LEAP-Seq n confirming: | 36852 |
| LEAP-Seq n nonconfirming: | 550 |
| LEAP-Seq n unique pos: | 122 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGCCGACTGTAATTGAG |
| Suggested primer 2: | CACGTACACGTACACCCCTG |