| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.077972 |
| Chromosome: | chromosome 9 |
| Location: | 3188035 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387319 | (1 of 2) PTHR11082//PTHR11082:SF25 - TRNA-DIHYDROURIDINE SYNTHASE // TRNA-DIHYDROURIDINE SYNTHASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTAGTTTGGGCTGGTATTTACAACCCAT |
| Internal bar code: | CTACGTCGTATAGGCCCCAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 577 |
| LEAP-Seq percent confirming: | 99.6564 |
| LEAP-Seq n confirming: | 290 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCCTGAGTGGAGCACAC |
| Suggested primer 2: | GCGTCGCTTCTTCATACACA |