Insertion junction: LMJ.RY0402.077976_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.077976
Chromosome:chromosome 9
Location:1926475
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g395150 MKP4,MKP3 (1 of 2) K14819 - dual specificity phosphatase 12 [EC:3.1.3.16 3.1.3.48] (DUSP12, YVH1); Dual-specificity protein phosphatase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTTTGGGCCCCGCAACGGCCACCTGCCC
Internal bar code:GCCAGAACTCTCTGATTGACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:175
LEAP-Seq percent confirming:99.5115
LEAP-Seq n confirming:1426
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTCTCCATGTCGTTGATG
Suggested primer 2:GTTACATGGGGATCATTCGG