| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.078033 |
| Chromosome: | chromosome_12 |
| Location: | 6135429 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g536000 | ALA2 | ATPase, aminophospholipid transporter | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGGGAGCGCCCGCCCCAACGAAGACCTTA |
| Internal bar code: | GCTCAGTCGTATGACCGCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 804 |
| LEAP-Seq percent confirming: | 99.4148 |
| LEAP-Seq n confirming: | 1529 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTACAGAACGCCGCACAC |
| Suggested primer 2: | GCCAGAGGGTAGGTGGTGTA |