Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.078033 |
Chromosome: | chromosome 12 |
Location: | 6135429 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g536000 | ALA2 | (1 of 3) K14802 - phospholipid-transporting ATPase [EC:3.6.3.1] (DRS2, ATP8A); ATPase, aminophospholipid transporter | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGAGCGCCCGCCCCAACGAAGACCTTA |
Internal bar code: | GCTCAGTCGTATGACCGCGCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 804 |
LEAP-Seq percent confirming: | 99.4148 |
LEAP-Seq n confirming: | 1529 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACTACAGAACGCCGCACAC |
Suggested primer 2: | GCCAGAGGGTAGGTGGTGTA |