| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.078083 |
| Chromosome: | chromosome 1 |
| Location: | 5827761 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041583 | (1 of 2) PF03366 - YEATS family (YEATS) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGGCTTCAAGCACACTCATGGCACAGC |
| Internal bar code: | GTGGTCCTCTTGAATAATGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 75 |
| LEAP-Seq percent confirming: | 83.2512 |
| LEAP-Seq n confirming: | 1690 |
| LEAP-Seq n nonconfirming: | 340 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTGGCTGTTGGACTTCT |
| Suggested primer 2: | GCTGTGGCGACTCTAGGAAC |