| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.078095 |
| Chromosome: | chromosome 2 |
| Location: | 5702421 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110050 | (1 of 1) PTHR22844:SF160 - WD40 REPEAT-CONTAINING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGTACGAGCGTGCACGCCGGCTGGCAC |
| Internal bar code: | ATGCTACGGGAGGCGGCGAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 591 |
| LEAP-Seq percent confirming: | 98.5855 |
| LEAP-Seq n confirming: | 10315 |
| LEAP-Seq n nonconfirming: | 148 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTAACTCGCTCGTCTCCGT |
| Suggested primer 2: | GTACACCGGGCTTGTCAGTT |