| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.078102 |
| Chromosome: | chromosome 5 |
| Location: | 2608401 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236250 | Conserved WD40-repeat Protein; (1 of 2) PTHR22847//PTHR22847:SF459 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGACCTCCTCCAGTCGGGCCTCCATAA |
| Internal bar code: | GACGCACGGTACTACGGACTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1052 |
| LEAP-Seq percent confirming: | 99.8248 |
| LEAP-Seq n confirming: | 4557 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTATTACATGCATGCCCA |
| Suggested primer 2: | ATGGCAAACAACCAAGGAAG |