| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.078140 |
| Chromosome: | chromosome 3 |
| Location: | 1519110 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g152100 | XRN2 | 5' to 3' exoribonuclease; (1 of 1) IPR004859//IPR027073//IPR029060 - Putative 5-3 exonuclease // 5'-3' exoribonuclease // PIN domain-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGTCGCAACATAAGCACGAATTCAGCTA |
| Internal bar code: | GGGGCGAGGTGGTAGCAAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 586 |
| LEAP-Seq percent confirming: | 99.209 |
| LEAP-Seq n confirming: | 878 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTCTTGGCCTTCTTCTT |
| Suggested primer 2: | AAACATGGCAGGTGGAGTTC |